Development of a vortex-assisted switchable-hydrophilicity solvent-based liquid phase microextraction for fast and reliable extraction of Zn (II), Fe (II), Pb (II), and Cd (II) from various baby food products.

This manuscript describes the development of a novel liquid phase microextraction (LPME) method for the extraction and determination of Zn (II), Fe (II), Pb (II), and Cd (II) in various infant/baby food and supplements products. The method is based on vortex-assisted extraction combined with a switchable-hydrophilicity solvent (SHS) sample preparation. The SHS, which undergoes reversible phase changes triggered by pH change, enables selective extraction and easy phase separation. A flame atomic absorption spectroscopy was used in the final determination step. Optimization studies revealed, that the optimal pH of the sample solution (after digestion) during analytes extraction is 5.5. A l-proline is added to the sample (375 mM) to ensure the complexation of the target metal cations. After the complexation step, 750 µL of SHS - a N, N-Dimethylcyclohexylamine along with 0.9 mL of 2 M of acetic acid solution is added (hydrophilicity switch-on stage) and mixed manually to obtain a homogeneous solution. In the last stage, 0.45 mL of 10 M NaOH solution (hydrophilicity switch-off stage) is added to the sample solution and a vortex for 100 s is applied to ensure the effective extraction and separation of the complex containing the analytes. At this stage, a cloudy solution is immediately obtained. Finally, the effective phase separation is obtained at the centrifugation step (4000 rpm for 2 mins). The method limit of detection was as 0.03, 0.009, 0.6, and 0.2 ng/L for Zn (II), Fe (II), Cd (II), and Pb (II) respectively with RSD% below 2.0 %. The analysis of certified reference materials and real samples proved the full applicability of the method for routine analysis, contributing to the field of heavy metal analysis and ensuring the safety of baby products. According to the AGREE methodology, this method can be named as green analytical chemistry method with a score of 0.77.

Assessing heavy metal levels in pediatric enteral nutrition formulas available in the Turkish market: Implications for consumer health.

In this study, we assessed the levels of lead (Pb), cadmium (Cd), mercury (Hg), and inorganic arsenic (iAs) in 27 pediatric enteral nutrition (EN) formulas from five international brands available in the Turkish market. Analysis was conducted using inductively coupled plasma mass spectrometry (ICP-MS). Non-carcinogenic and carcinogenic risk assessment was performed using hazard quotient (HQ), hazard index (HI), carcinogenic risk (CR), Toxicological contribution % of Provisional Tolerable Weekly Intake (PTWI) models. Our objective was to evaluate heavy metal exposure in EN formulas, specifically focusing on Cd, Pb, iAs, and Hg levels according to recommended amounts for different age groups based on their energy requirements. Average concentrations of iAs in polymeric (PC), oligomeric (OC), and monomeric (MC) EN formulas were as follows: PC: 2.13 ± 0.16 (

Determination of nitrate and nitrite levels in enteral nutrition formula marketed in Turkey: A risk assessment study.

In this study, the nitrite and nitrate levels of 37 enteral nutrition formulas of three different international brands marketed in Turkey were evaluated using high-performance liquid chromatography with diode-array detection (HPLC-DAD). Also, risk assessment of non-carcinogenic was calculated by models including hazard quotient (HQ) and hazard index (HI) in the deterministic model. Enteral nutrition formula consumption amounts obtained from individuals between the ages of 6-36 volunteers voluntarily participated in the study, and health risk assessments were calculated. The range of concentration of nitrate levels in enteral formulas of brands (B1, B2, and B3) was determined as 11.08 ± 2.88 (2.90-15.79), 11.64 ± 3.39 (2.92-22.93), and 10.66 ± 3.46 (4.92-15.37) mg/kg, respectively. The range of concentration of nitrite levels in enteral formulas of brands (B1, B2, and B3) was determined as 4.18 ± 1.10 (1.86-5.82), 3.70 ± 1.25 (1.29-5.26), and 3.38 ± 1.67 (2.00-5.29) mg/kg, respectively. The mean nitrate and nitrite levels of exposure from consumption of enteral nutrition formulas were determined as 0.14 and 0.11, 0.06 and 0.07 mg/kg bw/day for females and males, respectively. Nitrate and nitrite exposure levels calculated in the study did not exceed the ADI threshold values given by JECFA. The average HQ value calculated for the exposure of nitrate to male and female individuals was less than 1. Still, the P95 values calculated for nitrate were greater than 1.00 except for female and male individuals (24-36 ages). The HI value was found to be greater than 1.00 in all age groups of both genders. The presence of nitrites and nitrates in enteral nutrition formulas may lead to health problems in sensitive groups.

Estimation of exposure to phthalate esters from consumption of powdered infant formula sampled in Turkey.

Phthalate esters (PAEs) adversely affect the human endocrine and reproductive systems. These chemical toxic compounds are used as plasticizers, in particular to improve the mechanical properties of different food packing materials. Daily food consumption is the predominant source of exposure for PAEs, especially for infants. In this study, residue profiles and levels for eight PAEs were determined in 30 infant (stage I, II and special A and B) formulas of 12 brands in Turkey and health risk assessments were performed. The average level of PAEs were different for each formula group and packing type except for BBP (p < 0.01). The highest average mean level of PAEs was detected in paperboard type packing, while the lowest average mean level of PAEs was detected in metal can type packing. The highest average level of PAEs detected was DEHP in special formulas (22.1 ng g-1). The average hazard quotient (HQ) value was calculated as 8.43 × 10-5-8.94 × 10-5 for BBP, 1.49 × 10-3-1.58 × 10-3 for DBP, 2.06 × 10-2-2.18 × 10-2 for DEHP, and 7.21 × 10-4-7.65 × 10-4 for DINP. The average HI values were calculated as 2.29 × 10-2 for 0-6 months old infants, 2.39 × 10-2 for 6-12 months old infants, and 2.43 × 10-2 for 12-36 months old infants. These calculated results show that commercial infant formulas were a source of exposure to PAEs but did not present a significant health risk.

Analysis and health risk assessments of heavy metals and nitrate migration into pickle beverages.

This research aimed to investigate the levels of heavy metals (lead, cadmium, inorganic arsenic, and aluminium) and nitrate contaminants in pickle beverages sold in the Turkey market produced from various fruits and vegetables. In addition, carcinogenic and noncarcinogenic risk assessments of exposure resulting from oral consumption of these beverages have also been made. The heavy metal concentrations in 22 pickle beverages were found to be in the range of 0.369 to 119.181 for Al, 0.136 to 6.561 for iAs, 0.020 to 1.326 for Cd, and 0.118 to 3.632 µg/L for Pb, while the nitrate concentrations were < limits of detection (LOD) 117.320 mg/L. Hazard quotient values for heavy metals and nitrate are calculated as <1 in both genders and are safe regarding noncarcinogenic risks. Lifetime carcinogenic risk assessment indicated that some pickle beverages might pose a carcinogenic risk at grade A (>10-4) for iAs and grade B (10-6-10-4) for Pb in both genders.